LabReport (#419)

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# CERTIFICATE OF ANALYSIS

**SAMPLE NAME:** GOLDEN GROVE - BLUEBERRY
**SAMPLE CODE:** DTS-240122-006
**CLIENT:** PICCO BEVERAGES
**BATCH RESULT:** PASS

**MATRIX NAME:** Beverage
**SAMPLE TYPE:** Quality Assurance
**RECEIVED DATE:** Mon, Jan 22, 2024
**PUBLISHED DATE:** Thu, Jan 25, 2024
**BATCH CODE:**
**BATCH SIZE:** 0
**SAMPLE SIZE:** 5U
**AVERAGE UNIT WEIGHT:** 228.83g (1 can)

**TOTAL MAJOR ALKALOIDS:** 37.30 mg/serv
**TOTAL MINOR ALKALOIDS:** ND
**MITRAGYNINE:** 29.06 mg/serv

| ALKU | Kratom Alkaloids<br/>High Level | SAL | Salmonella<br/>GeneUp qPCR 25g | ECOLI | Total E. Coli & Coliforms<br/>Plate | TAMC | Total Aerobic Bacteria<br/>Plate |
| ----- | -------------------------------- | ----- | ------------------------------ | ----- | ------------------------------------ | ----- | ------------------------------------------------ |
| ALKL | Kratom Alkaloids<br/>Low Level | BRX | Brix of Liquids | DEN | Density of Liquids | COOX | Carbon Dioxide &<br/>Dissolved Oxygen of Liquids |
| PH | pH & Electric Conductivity (EC)z | TURB | Turbidity<br/>Liquids | SAUR | Staphylococcus aureus<br/>3M Plating | LMON | Listeria monocytogenes<br/>GeneUp qPCR |
| DIADT | Opioid Adulterants | CAFFE | Caffeine | TMYFD | Total Yeast & Mold<br/>Plate | ENTRO | Enterobacteriaceae<br/>Plate |
| HPMET | Heavy Metals<br/>? | | | | | | |


**RESULTS REVIEWED BY:** Leslie Varela
Laboratory Director
Cambium Analytica
Thursday, Jan 25, 2024

**RESULTS CERTIFIED BY:** Douglas Smith
VP - Scientific Operations
Cambium Analytica
Thu, Jan 25, 2024

This report may not be reproduced except in full without approval from Cambium Analytica. The results herein relate only to the sample & batch identified in this report.
---
# LAB-TM-052 - Determination of Kratom Alkaloid Content by UPLC-DAD
**ALKU-DTS-240122-006-01 - Mon, Jan 22, 2024**

| Analyte | Value | Value (Per Serving) | Value (Per Package) | Action Limit | LOD | LOQ | Status |
|-----------------------|--------|---------------------|---------------------|--------------|-----------|-----------|--------|
| Total Major Alkaloids | 0.016% | 37.30 mg | 37.30 mg | N/A | 0.3 ug/g | 0.5 ug/g | N/A |
| Mitragynine | 0.012% | 29.06 mg | 29.06 mg | N/A | 0.3 ug/g | 0.5 ug/g | N/A |
| Speciociliatine | 0.002% | 3.89 mg | 3.89 mg | N/A | 0.3 ug/g | 0.5 ug/g | N/A |
| Paynantheine | 0.001% | 2.75 mg | 2.75 mg | N/A | 0.3 ug/g | 0.5 ug/g | N/A |
| Speciogynine | 0.001% | 1.60 mg | 1.60 mg | N/A | 0.3 ug/g | 0.5 ug/g | N/A |

# LAB-TM-047 - Determination of Kratom Alkaloid Content by LC-TQ
**ALKU-DTS-240122-006-01 - Mon, Jan 22, 2024**

| Analyte | Value | Value (Per Serving) | Value (Per Package) | Action Limit | LOD | LOQ | Status |
|-----------------------|-------|---------------------|---------------------|--------------|--------------|--------------|--------|
| 7-Hydroxymitragynine | ND | ND | ND | N/A | 0.01 ug/kg | 0.1 ug/kg | N/A |
| Total Minor Alkaloids | ND | ND | ND | N/A | 0.01 ug/kg | 0.1 ug/kg | N/A |
| Mitraphylline | ND | ND | ND | N/A | 0.01 ug/kg | 0.1 ug/kg | N/A |

# LAB-TM-063 - Detection and Isolation of Salmonella spp. in Foods and Dietary Supplements
**SAL-DTS-240122-006-01 - Mon, Jan 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|---------------|-------|--------------|-----|-----|--------|
| Salmonella Spp | ND | N/A | | | N/A |

# LAB-TM-059 - Enumeration of Escherichia Coli and Total Coliform in Foods and Dietary Supplements
**ECOIL-DTS-240122-006-01 - Mon, Jan 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|---------------|-----------|--------------|-----|-----|--------|
| E. coli | <10 CFU/g | N/A | | | N/A |
| Total Coliforms | <10 CFU/g | N/A | | | N/A |

# LAB-TM-060 - Enumeration of Total Aerobic Count in Foods and Dietary Supplements
**TAMC-DTS-240122-006-01 - Mon, Jan 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|--------------------|-----------|--------------|-----|-----|--------|
| Total Aerobic Count | <10 CFU/g | N/A | | | N/A |

# LAB-TM-017 - Brix & Density Analysis
**BRX-DTS-240122-006-01 - Mon, Jan 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|---------|--------|--------------|-----|-----|--------|
| Brix | 11.1 bx | N/A | | | N/A |

# LAB-TM-017 - Brix & Density Analysis
**DEN-DTS-240122-006-01 - Mon, Jan 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|---------|----------|--------------|-----|-----|--------|
| Density | 1.043 g/mL | N/A | | | N/A |

# LAB-TM-020 - Determination of Carbon Dioxide and Dissolved Oxygen in Beverages
**COOX-DTS-240122-006-01 - Mon, Jan 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|-----------------------|-------------|--------------|-----|-----|--------|
| Dissolved Carbon Dioxide | 1.89 vol | N/A | | | N/A |
| Dissolved Oxygen | 16.30 ug/kg | N/A | | | N/A |

---
# DTS-240122-006

## LAB-TM-018 - TEST METHOD FOR DETERMINING PH
**PH-DTS-240122-006-01 - MON, JAN 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|---------|-------|--------------|-----|-----|--------|
| pH | 3.707 | N/A | N/A | N/A | N/A |

## LAB-TM-021 - TURBIDITY BY UV-VIS
**TURB-DTS-240122-006-01 - MON, JAN 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|-----------|----------|--------------|-----|-----|--------|
| Turbidity | 60.584% | N/A | N/A | N/A | N/A |

## LAB-TM-062 - ENUMERATION OF STAPHYLOCOCCUS AUREUS IN FOODS AND DIETARY SUPPLEMENTS
**SAUR-DTS-240122-006-01 - MON, JAN 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|------------|-------------|--------------|-----|-----|--------|
| S. aureus | <10 CFU/g | N/A | N/A | N/A | N/A |

## LAB-TM-064 - DETECTION AND ISOLATION OF L. MONOCYTOGENES IN FOODS AND DIETARY SUPPLEMENTS
**LMON-DTS-240122-006-01 - MON, JAN 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|----------------------|-------|--------------|-----|-----|--------|
| Listeria monocytogenes | ND | N/A | N/A | N/A | N/A |

## LAB-TM-074 - QUALITATIVE DETERMINATION OF PAIN MANAGEMENT ADULTERANTS IN BOTANICAL PRODUCTS
**DTADT-DTS-240122-006-01 - MON, JAN 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|------------------------|-------|--------------|-----------|-----|--------|
| 4-Fluoro-Isobutyryl Fentanyl | ND | Detection | 1.20 ug/g | | PASS |
| Acetyl Fentanyl | ND | Detection | 0.48 ug/g | | PASS |
| Benzoyl Fentanyl | ND | Detection | 0.48 ug/g | | PASS |
| Buprenorphine | ND | Detection | 4.80 ug/g | | PASS |
| Butyryl Fentanyl | ND | Detection | 1.20 ug/g | | PASS |
| Carfentanyl | ND | Detection | 1.20 ug/g | | PASS |
| Codeine | ND | Detection | 4.80 ug/g | | PASS |
| Cyclopropyl Fentanyl | ND | Detection | 0.48 ug/g | | PASS |
| Fentanyl | ND | Detection | 0.48 ug/g | | PASS |
| Furanyl Fentanyl | ND | Detection | 0.48 ug/g | | PASS |
| Heroin | ND | Detection | 4.80 ug/g | | PASS |
| Hydrocodone | ND | Detection | 4.80 ug/g | | PASS |
| Hydromorphone | ND | Detection | 4.80 ug/g | | PASS |
| Meperidine | ND | Detection | 4.80 ug/g | | PASS |
| Methadone | ND | Detection | 4.80 ug/g | | PASS |
| Morphine | ND | Detection | 4.80 ug/g | | PASS |
| Oxycodone | ND | Detection | 4.80 ug/g | | PASS |
| Oxymorphone | ND | Detection | 4.80 ug/g | | PASS |
| Tramadol | ND | Detection | 4.80 ug/g | | PASS |

---
# DTS-240122-006

## LAB-TM-076 - DETERMINATION OF CAFFEINE AND MELATONIN BY HPLC
**CAFFE-DTS-240122-006-01 - MON, JAN 22, 2024**

| Analyte | Value | Value (Per Serving) | Value (Per Package) | Action Limit | LOD | LOQ | Status |
|----------|-----------|---------------------|---------------------|--------------|-----------|----------|--------|
| Caffeine | 0.0574 mg/g | 13.13 mg | 13.13 mg | N/A | 0.025 mg/g | 0.05 mg/g | N/A |

## LAB-TM-061 - ENUMERATION OF YEAST AND MOLD IN FOODS AND DIETARY SUPPLEMENTS
**TYMFD-DTS-240122-006-02 - MON, JAN 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|-------------------|------------|--------------|-----------|----------|--------|
| Total Yeast and Mold | <10 CFU/g | N/A | N/A | N/A | N/A |

## LAB-TM-084 - ENUMERATION OF ENTEROBACTERIACEAE
**ENTRO-DTS-240122-006-02 - MON, JAN 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|--------------------|------------|--------------|-----------|----------|--------|
| Enterobacteriaceae | <10 CFU/g | N/A | 100 CFU/g | 100 CFU/g | N/A |

## LAB-TM-044 - DETERMINATION OF HEAVY METALS BY ICP-MS
**HPMET-DTS-240122-006-01 - MON, JAN 22, 2024**

| Analyte | Value | Action Limit | LOD | LOQ | Status |
|----------|-----------|--------------|------------|------------|--------|
| Arsenic | ND | N/A | 0.1985 µg/kg | 0.6616 µg/kg | N/A |
| Cadmium | ND | N/A | 0.0817 µg/kg | 0.2724 µg/kg | N/A |
| Chromium | 0.004 µg/g | N/A | 0.6272 µg/kg | 2.0907 µg/kg | N/A |
| Copper | 0.060 µg/g | N/A | 0.1546 µg/kg | 0.5152 µg/kg | N/A |
| Lead | 0.006 µg/g | N/A | 0.2817 µg/kg | 0.2817 µg/kg | N/A |
| Mercury | 0.001 µg/g | N/A | 0.0171 µg/kg | 0.0571 µg/kg | N/A |
| Nickel | 0.006 µg/g | N/A | 0.3213 µg/kg | 1.0709 µg/kg | N/A |